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Extra info for Calcified Tissues 1975: Proceedings of the XIth European Symposium on Calcified Tissues

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80 w"'tl ,_ z c :> 0~ u 0 :; ~ 60 u"..!! g Ca/mg pratein) Figure 7. CaBP content of mucosal scrapings as a function of their calcium content. Mucosal scrapings of male Sprague-Dawley rats were homogenized and centrifuged (100,000 g). The supemate (initial supemate) was analyzed for calcium and protein content and subjected to gel chromatography (4). Peak B of the eluate was then analyzed for CaBP. Each point is based on material from 6 rats. Eight different experiments were done over a span of 18 months.

10), but detected CaBP in cells that had been treated with 1,25-dihydroxy-vitamin D3 in vitro (panel C, Fig. 10). These measures have since been quantitated (16). 1 Qi ... ö. w ""~ Q.. ::::> ::E ::::> ü tJ. 4. V 0 1,25- (OH~ -03 • 25-0H- D3 0 VEHlCLE 3 TIME, 4 5 MINUTE S Figure 9. The effect of the addition of 1,25-dihydroxy-vitamin D 3 to the isolation medium on calcium uptake by rat intestinal cells. Cells were harvested and assayed as described in the legend, Fig. 7 x 10-BM 25-(0H)-D3. The remainder of the strips were placed in an isolation medium that contained a control volume of a 1:1 mixture of propylene giycol and ethanol ("vehicle").

Dupuis, Y. : Effets differents de Ia Vitamine D et du Iactose sur le rat traite par l'actinomycine D en cours de carence fonctionnelle du calcium. R. Soc. Biol. (Paris) 163, 2530-2533 (1969) 5. : Lactose and intestinal absorption of calcium. Investigative UroL 7, 433-441 (1970) 6. : L'effet protecteur du Iactose vis a vis du squelette de Ia ratte allaitante. R. Acad. Sei. (Paris} 238, 509-511 (1954) 7. Fournier, P. : Pouvoir antirachitique de composes divers dits de structure Iactose, glucosamine, L xylose, mannitol.

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